期刊论文

Study on the detection of heavy metals in rice using luminescence bacteria

中文

粮食与油脂

1008-9578

2016

29

5

63-66

10.3969/j.issn.1008-9578.2016.05.018

:农业部财政部农办财函(2015)38号 :省农委财政厅湘农联(2015)112号

本校为第一机构

以明亮发光杆菌（P.phosphoreum）为受试物,对大米中的铅、镉、汞三种重金属进行了检测。结果表明,发光抑制率与重金属溶液的质量浓度呈正相关,相关系数大于0.96,各重金属离子对P.phosphoreum的毒性大小排序为Hg^2＋〉Pb^2＋〉Cd^2＋。菌液与各重金属标准溶液加样体积比为1∶1,检测时间为30 min;Hg Cl2的线性范围为0.02~1.6 mg/L,Pb（NO_3）_2的线性范围为2~20 mg/L,Cd Cl_2的线性范围为2~64 mg/L;样品加标回收率范围为70.12%~93.5%,相对标准偏差范围为5.31%~13.25%。该检测方法简便,成本低,精确度较高,测量结果直观。

The lead,cadmium and mercury were detected using luminescent bacteria test. The results showed that the light inhibition rate was positively correlated with the concentration of heavy metal solution,and the correlation coefficient was higher than 0.96. The list of the toxicity sequence was Hg~（2＋）Pb~（2＋）Cd~（2＋）.Bacteria and heavy metals standard solution was loaded volume ratio of 1∶1,detection time was 30 min. The linear working range was 0.02~1.6,2~20,2~64 mg/L for Hg Cl_2,Pb（NO_3）_ 2 and Cd Cl_2. Spiked tests revealed that recove...