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Functional three helix molecular beacon fluorescent “turn-on” probe for simple and sensitive simultaneous detection of two HIV DNAs

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成果类型:
期刊论文
作者:
Han, Yunpeng;Zhang, Feng;Gong, Hang*;Cai, Changqun*
通讯作者:
Gong, Hang;Cai, Changqun
作者机构:
[Cai, Changqun; Gong, Hang; Cai, CQ; Han, Yunpeng] Xiangtan Univ, Coll Chem, Key Lab Green Organ Synth & Applicat Hunan Prov, Key Lab Environm Friendly Chem & Applicat,Minist, Xiangtan 411105, Peoples R China.
[Zhang, Feng] Hunan Agr Univ, Coll Sci, Changsha 410128, Hunan, Peoples R China.
通讯机构:
[Gong, H; Cai, CQ] X
Xiangtan Univ, Coll Chem, Key Lab Green Organ Synth & Applicat Hunan Prov, Key Lab Environm Friendly Chem & Applicat,Minist, Xiangtan 411105, Peoples R China.
语种:
英文
关键词:
Three helix molecular beacons (tMBs);Simultaneous detection HIV DNAs;Single-labeled;G-quadruplex
期刊:
Sensors and Actuators B-Chemical
ISSN:
0925-4005
年:
2019
卷:
281
页码:
303-310
基金类别:
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [21775132, 21305118, 21505112]; Scientific Research Foundation of Hunan Provincial Education DepartmentHunan Provincial Education Department [16A204]; National Natural Science Foundation of Hunan provinceNatural Science Foundation of Hunan Province [2018JJ2388]
机构署名:
本校为其他机构
院系归属:
理学院
摘要:
A fluorescent biosensing strategy based on three helix molecular beacons (tMBs) for simultaneous simple and rapid detection of HIV related genes was developed. This tMBs contain outer loop sequences and fluorescent probes. The outer loop sequences consist of target capture sequences (cDNA) and the stems can lock the fluorescent probes (P). Initially, the probes were tethered to display weak fluorescence signals in the tMBs. Once the target DNAs opened loops, the stems of tMBs were dehybridized which resulted in turning on the fluorescence. By applying tMBs fluorescent switch detection strategy...

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