期刊论文

Gong, Hang;Cai, Changqun

英文

Sensors and Actuators B-Chemical

0925-4005

2019

281

303-310

10.1016/j.snb.2018.10.110

National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [21775132, 21305118, 21505112]; Scientific Research Foundation of Hunan Provincial Education DepartmentHunan Provincial Education Department [16A204]; National Natural Science Foundation of Hunan provinceNatural Science Foundation of Hunan Province [2018JJ2388]

本校为其他机构

A fluorescent biosensing strategy based on three helix molecular beacons (tMBs) for simultaneous simple and rapid detection of HIV related genes was developed. This tMBs contain outer loop sequences and fluorescent probes. The outer loop sequences consist of target capture sequences (cDNA) and the stems can lock the fluorescent probes (P). Initially, the probes were tethered to display weak fluorescence signals in the tMBs. Once the target DNAs opened loops, the stems of tMBs were dehybridized which resulted in turning on the fluorescence. By applying tMBs fluorescent switch detection strategy...