Efficient ASK-assisted system for expression and purification of plant F-box proteins

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成果类型：

期刊论文

作者：

Li, Haiou;Yao, Ruifeng;Ma, Sui;Hu, Shuai;Li, Suhua;...

通讯作者：

Xie, Daoxin;Yan, Jianbin

作者机构：

[Li, Suhua; Yan, Jianbin; Wang, Yupei; Xie, Daoxin; Li, Haiou; Yan, Chun; Yao, Ruifeng; Ma, Sui; Hu, Shuai] Tsinghua Univ, MOE Key Lab Bioinformat, Tsinghua Peking Joint Ctr Life Sci, Sch Life Sci, Beijing 100084, Peoples R China.

[Li, Haiou] Hunan Agr Univ, Hunan Prov Key Lab Phytohormones & Growth Dev, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Changsha 410128, Hunan, Peoples R China.

[Yan, Jianbin] Tsinghua Univ, Ctr Synthet & Syst Biol, Beijing 100084, Peoples R China.

通讯机构：

[Xie, DX; Yan, JB; Yan, Jianbin] T

Tsinghua Univ, MOE Key Lab Bioinformat, Tsinghua Peking Joint Ctr Life Sci, Sch Life Sci, Beijing 100084, Peoples R China.

Tsinghua Univ, Ctr Synthet & Syst Biol, Beijing 100084, Peoples R China.

语种：

英文

关键词：

COI1;SLY1;TIR1;ASK;F-box protein;Technical Advance

期刊：

PLANT JOURNAL

ISSN：

0960-7412

年：

2017

卷：

期：

页码：

736-743

DOI：

10.1111/tpj.13708

基金类别：

National Key R&D Program of China [2016YFA0500501]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31500228]; Tsinghua-Peking Joint Center for Life Sciences; China Association for Science and Technology

机构署名：

本校为其他机构

院系归属：

生物科学技术学院

摘要：

Ubiquitin-mediated protein degradation plays an essential role in plant growth and development as well as responses to environmental and endogenous signals. F-box protein is one of the key components of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which recruit specific substrate proteins for subsequent ubiquitination and 26S proteasome-mediated degradation to regulate developmental processes and signaling networks. However, it is not easy to obtain purified F-box proteins with high activity due to their unstable protein structures. Here, we found that Arabidopsis SKP-like pr...

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Efficient ASK-assisted system for expression and purification of plant F-box proteins

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