期刊论文

中文

干旱地区农业研究

1000-7601

2008

26

3

109-114,123

中国博士后科学基金(第三十九批)； 河南省教育厅自然科学基金（2006210007）；

本校为其他机构

采用RT-PCR方法从小麦品种豫教2号的籽粒中克隆出淀粉合酶Ⅱ基因(Starch synthase Ⅱ, SSⅡ)部分cDNA片段(600bp) (GenBank No.EF221761),同源性比较结果显示,它与GenBank上已报道的SSⅡ基因有高度同源性.以pCMBIA1301质粒为基础,构建了由35S启动子调控的SSⅡ基因的反义表达载体pCMBIA1301SSIIA;另外,还以pFGC5941质粒为基础,构建了SSⅡ基因的RNAi载体pFGC5941SSIIsa,这些载体的构建为研究此基因的功能奠定了基础.

Starch synthase Ⅱ （SS Ⅱ ） partial eDNA sequences （600bp） （GenBank No. EF221761） from grains of common wheat （ Triticum aestivum, Yujiao 2 cultuvar） was amplified by RT-PCR. The result demonstrated that the cloned SS Ⅱ gene sequences were 99 % identified with the reported SS Ⅱ genes in GenBank previously. In addition, its antisense expression vector was constructed with pCMBIA1301, and RNAi vector was also constructed with pFGC5941. These constructed vectors will provide a good background to study the function of S...