期刊论文

中文

中国环境监测

1002-6002

2017

33

3

139-146

10.19316/j.issn.1002-6002.2017.03.21

环保公益性行业科研专项项目（201509038）；

本校为第一机构

建立高浓度有机废水中5种喹诺酮类抗生素的高效液相色谱-串联质谱测定方法。水样经HLB固相萃取小柱富集净化,12 ml甲醇洗脱、浓缩并加入内标溶液后,定容至1 mL待测。以C_(18)柱为分离柱,含0.01%甲酸的甲醇-含0.01%甲酸的水溶液为流动相,目标物质在10 min内分离。在0.25 ~ 1 250 ng/mL范围内,目标物质线性关系良好(R~2 >0.99)。基质加标试验结果表明,纯水中的回收率为61.40% ~91.92%,废水中的回收率为54.92% ~101.87%,检出限为0.25~2.5 ng/L,方法定量限为0.36~3.99 ng/L。应用该方法对21家猪场的64份废水样品进行分析,5种喹诺酮类抗生素的检出频率为47% ~95%,平均检出浓度为980~5 734 ng/L。该方...

A method was established for determination of 5 fluoroquinolone antibiotics in high concentration of organic wastewater using solid phase extraction ( SPE) followed by high performance liquid chromatography-tandem mass spectrometry ( HPLC-MS/ MS) in this paper. Aqueous samples were developed with Oasis HLB solid-phase extraction ( SPE) cartridges and the targeted antibiotics were subsequently eluted by 12 mL methanol. After concentration, the elutes were added internal standards and determined by HPLC-MS/MS equipped with a reverse-phase C18 column. The results of method development showed that...