期刊论文

Huang, R.-S.

中文

中国中药杂志

1001-5302

2005

30

13

971-977

10.3321/j.issn:1001-5302.2005.13.003

2001CB108905:国家重点基础研究发展计划（973计划）

本校为其他机构

目的:研究鸡骨草根瘤菌的生物学特性.方法:将来自不同生态环境的鸡骨草根瘤经分离纯化获得根瘤菌菌株,再进行生长特性、增代时间以及对温度,pH,NaCl和抗生素的耐受性试验.结果:原产亚热带的鸡骨草根瘤菌在YMA培养基上代谢产碱,平均增代时间为14.8 h,应属慢生型根瘤菌;来源不同生态环境的参试菌株在对高温的忍耐能力、酸性环境的适应性、抗生素的敏感性等多种抗逆特性方面存在较大的差异,但对碳源和氮源的利用特性比较一致;接种根瘤菌70d后,鸡骨草幼苗的结瘤率为85.0%,茎叶干物质增加51.1%.结论:鸡骨草根瘤菌存在较为丰富的根瘤菌抗高温和抗酸性环境的种质资源,为今后在生产上利用鸡骨草根瘤菌资...

Objective: The research aimed at studying the biological characteristics of rhizobia isolated from Abrus cantoniensis. Method: The rhizobia strains, isolated from different environments in Guangxi, were studied for their growing characters and the generation time. They were also compared for survival capabilities under stresses caused by NaCl, pH, temperature, and different kinds and concentration of antibiotics. Result: The strains obtained from A. cantoniensis in subtropical zone produced alkali in YMA medium, the average generation time was 14.8 hours, and thus they belong to slow-growing r...