期刊论文

中文

植物病理学报

0412-0914

2007

37

3

301-306

10.3321/j.issn:0412-0914.2007.03.014

湖南省教育厅重点资助项目（02A019）；

本校为第一机构

在烟草青枯病区采取健康烟草植株，从其茎杆内分离到2株对烟草青枯拉尔氏菌（Ralstonia solanacarum）有强拮抗作用的内生菌株009和011。形态观察、生理生化鉴定及16S rDNA序列比对结果表明，菌株009和011均归属为Brevibacillus brevis，009、011菌株与B．brevis（AY591911）相似性分别为99．5％和99．0％，GenBank登录号分别为DQ444284、DQ444285。生长特性研究结果表明，它们的最适生长pH值分别为6．5、7．5，最适生长温度分别为25、30℃。温室内用淋根法分别先接种009和011菌株，后接种病原菌，其防效分别为87．25％和52．30％。用009和011菌液分别和烟草青枯病菌的混合液淋根，其防效明显低...

Endophytic bacterial strains 009 and 011 isolated from the stems of the health tobacco growing at infected fields strongly inhibited growth of Ralstonia solanacarum, the pathogen of tobacco bacterial wilt. Based on morphological, physiological, biochemical characteristics, and 16S rDNA phylogenetic analysis, strains 009 and 011 were identified as Brevibacillus brevis. High similarities of 16S rDNA sequence between strains 009 and 011with B. brevis （AY591911） were 99.5% and 99.0% respectively. The GenBank accession numbers of are DQ444284 for ...