期刊论文

Analysis of cpDNA Sequence of Kinship among Different Strains of Jianghua Kucha

中文

茶叶通讯

1009-525X

2017

44

3

22-26

国家现代农业产业技术体系建设专项（CARS-23）； 湖南省农业科技创新项目（2017JC15）；

本校为第一机构

DNA序列分析在物种的系统进化、分类和鉴定等方面展示出了强大的生命力,其中cpDNA序列分析因具有基因组小、进化速率适中、母系遗传等特点,已被大量用于系统进化研究.本研究采用4对cpDNA引物对收集来的32份江华苦茶资源进行了亲缘关系研究,其中有3对测序成功.扩增序列长度分别为634(1F-724R)、499(rbcla-rev)、532(rbcla-ajf634).变异位点数量分别为5(1F-724R)、4(rbcla-rev)、4(rbcla-ajf634).变异率从高到低分别是rbcla-rev(0.80%)、1F-724R(0.79%)、rbcla-ajf634(0.75%).将3对引物测序得到的序列拼接,按照MP法构建了分子系统树,将参试的32份茶树资源分为4大类.本研究为江华苦茶亲缘关系研究开...

DNA sequence analysis has shown a strong vitality in the phylogenetic evolution, classification and identification of species. The cpDNA sequence analysis has been widely used in phylogenetic studies because of its small genome, moderate evolution rate and maternal inheritance. In this study, four pairs of cpDNA primers were used to study the genetic relationship among 32 ,lianghua Kucha resources. Three of them were successfully sequenced. Amplification sequence length was 634 （1F-724R）, 499 （rbcla-rev）, 532 （rbcla-ajf634）, respectively....