期刊论文

Study on the Separation of Anthocyanins in Purple Bud tea by High-Speed Counter-current Chromatography coupled with Semi-Preparative High Performance Liquid Chromatography

中文

茶叶通讯

1009-525X

2019

46

2

192-200

10.3969/j.issn.1009-525X.2019.02.012

国家重点研发计划(2017YFD0400803) 湖南省科技重大专项(2017NK1020) 长沙市科技重大专项(kq1804003).

本校为第一机构

花色苷是植物中一类具有重要生物活性的次生代谢产物,因其含量较低,使得分离制备花色苷高纯品难度较大.本研究以茶树紫芽为实验材料,以花色苷得率与纯度为考察指标,优化筛选了应用高速逆流色谱结合半制备型液相色谱分离纯化花色苷高纯度的方法.结果表明,茶样经提取、脱脂、阳离子交换树脂分离等前处理后,总花色苷得率28.18 mg/g,纯度为20.38％;以高速逆流色谱分离的最优溶剂体系乙酸乙酯:正丁醇:乙腈:0.1％ 三氟乙酸水溶液=8:35:13:60(v/v/v/v),除去部分杂质后,总花色苷得率达到160.59 mg/g,纯度为43.64％,再结合半制备型液相色谱分离出5种花色苷组分,纯度最高达90.61％,其优化色谱条件为C18制备...

Anthocyanins are a class of secondary metabolites in plants with important biological activities.it is difficult to separate and prepare high-purity anthocyanins from plants because of the low-content.the purple bud tea was used as experimental material in this study,taking the yield and the purity of anthocyanins as an indicators,the method of separation and purification of anthocyanins by high-speeed counter-current chromatography(HSCCC)coupled with semipreparative high performance liquid chromatography(PHPLC)were optimized and screened.The r...