期刊论文

中文

茶叶通讯

1009-525X

2019

46

2

162-169

10.3969/j.issn.1009-525X.2019.02.008

2018NK2033:湖南省重点研究计划项目 kq1801023:长沙市科技计划 kq1701038:长沙市科技计划 18A090:湖南省教育厅重点项目

本校为第一机构

分别选取有机茶园(OTP)、无公害茶园(NPTP)和普通茶园(CTP)的茶树根际土壤,利用Illumina Miseq高通量测序技术研究其细菌群落多样性、结构和组成,并分析茶园土壤理化性质与细菌群落结构的相关性。结果表明:有机茶园土壤的细菌群落多样性指数(Chao1)显著高于无公害和普通茶园土壤;3种茶园土壤细菌群落结构存在显著性差异,变形菌门(Proteobacteria)、酸杆菌门(Acidobacteria)和绿弯菌门(Chloroflexi)在不同茶园土壤中都属于优势菌群,但有机茶园土壤的细菌群落组成在门和属水平上与无公害和普通茶园存在显著差异;土壤有效磷(AP)、总磷(TP)和pH值是影响茶园土壤细菌群落结构的关键理化因子。

Illumina Miseq high-throughput sequencing technology was used to study the soil bacterial community diversity,structure and composition of organic tea plantation(OTP),non-polluted tea plantation(NPTP)and common tea plantation(CTP).Alpha-diversity index(Chao1)of OTP was significantly higher than that of NPTP and CTP.PCoA results showed the clear separation of bacterial communities structure in the three tea plantations soils.The results of relative bacterial abundance showed that Proteobacteria,Acidobacteria and Chloroflexi were most dominant bacteria in all tea plantation soil samples under th...