期刊论文

中文

茶叶通讯

1009-525X

2019

46

2

141-148

10.3969/j.issn.1009-525X.2019.02.005

国家现代农业产业技术体系(CARS-19) 湖南省科技重点研发项目(2018NK2031) 湖南省农业科技创新项目(2017JC15).

本校为第一机构

采用荧光SSR分子标记与cpDNA序列分析技术相结合的方法,对江华苦茶4个自然居群的32份资源进行遗传多样性、亲缘关系和遗传分化研究.结果表明:cpDNA变异率约为0.78％,分为5个单倍型,单倍型多样性为Hd=0.726,核苷酸多样性为 π=0.00233,遗传多样性较高;H3是基础的单倍型,H4和H5之间只有一个位点的差异,亲缘关系最近;居群遗传分化系数较高(FST=0.26019),基因流较低(Nm=0.71),cpDNA变异主要在居群内(89.29％),居群间遗传分化程度较低;中性检测和失配分析结果表明江华苦茶没有发生扩张事件.荧光SSR分子标记Ho、He和Nei分别为0.56、0.65和0.64,遗传多样性较高;3号和27号样品与其他样品相似度低于75％,...

The genetic diversity,genetic relationship and genetic differentiation of 32 natural resources of Jianghua bitter tea were studied by the combination of fluorescent SSR molecular markers and cpDNA sequence analysis.The results showed that the cpDNA mutation rate was about 0.78%,which was divided into five haplotypes.The haplotype diversity was Hd=0.726,the nucleotide diversity wasπ=0.00233,and the genetic diversity was higher.H3 was the basic single.There is only one locus difference between H4 and H5,and the phylogenetic relationship is the c...