期刊论文

中文

中国兽医科学

1673-4696

2014

44

1

21-26

10.16656/j.issn.1673-4696.2014.01.010

公益性行业（农业）科研专项（201303037） 甘肃省创新研究群体计划项目（1210RJIA006）

本校为其他机构

本研究的目的是通过对绵羊夏柏特线虫和叶氏夏柏特线虫线粒体细胞色素b基因(cytb)部分序列(pcytb)和烟酰胺嘌呤二核苷酸(NADH)脱氢酶亚单位5基因(nad5)部分序列(pnad5)的克隆和遗传变异分析,首次为在分子水平上区分绵羊夏柏特线虫和叶氏夏柏特线虫提供理论依据。采用PCR方法分别克隆了6条绵羊夏柏特线虫陕西分离株和9条叶氏夏柏特线虫青海分离株的pcytb和pnad5。将测序结果应用ClustalX 1.81程序进行比对,然后用PAUP 4.0程序中的MP法将pcytb和pnad5串联后构建系统发育树。结果显示,获得的pnad5和pcytb序列的长度分别为388bp和659bp,其变异率在绵羊夏柏特线虫分离株中分别为0.3%~1.3%和0.2%~...

The present study aimed tO examine sequence variations in cytochrome b（cytb） and NADH dehydrogenase submit 5（nad5） genes among Chabertia ovina isolates from goats in Shaanxi Province and Chabertia erschowi isolates from yaks in Qinghai Province, and to study the phylogenetic relationship of the two species based on two genes. The partial cytb（pcytb） and nadS（pnadS） were amplified from individual samples and then sequenced. The obtained sequences were aligned using the ClustalX 1.81. Then MP tree was constructed using PAUP 4. 0. The leng...