期刊论文

中文

中国兽医科学

1673-4696

2014

44

1

39-44

10.16656/j.issn.1673-4696.2014.01.004

湖南省科技计划项目

本校为第一机构

采用PCR方法对湖南省分离的42株粪肠球菌的8种毒力相关的基因即溶血素基因cylA、明胶酶基因gelE、表面蛋白基因esp、胶原蛋白黏附素基因ace、聚集物质基因asa1、心内膜炎有关的抗原基因efaA以及2个假定毒力相关基因EF3314、EF0591进行了检测,并用平板法测定了这些分离株的溶血性和明胶溶解性。结果显示,24株粪肠球菌分离株普遍存在EF3314基因,检出率为100%;其次是efaA基因,检出率为92.9%;其他毒力基因gelE、ace、asa1、cylA、esp、EF0591的检出率为分别为88.1%、59.5%、52.4%、54.8%、38.1%、4.76%。菌株在绵羊血琼脂平板上以α溶血为主,而在家兔血琼脂平板中以β溶血为主,家兔血琼脂平板溶血的...

Eight virulence associated genes were detected from 42 strains of Enterococcus faecalis by PCR in Hunan Province,and the hemolytic and gelatin solubility of these isolates were also determined by plate method. The 8 genes included cytolysin activator gene cylA, gelatinase gene gelE,Enterococcus sur- face protein gene esp, collagen adhesion gene ace, aggregation substance gene asal,E, faecalis endocarditis antigen gene efaA,and 2 supposed virulence associated genes EF3314 and EF0591 were also included. In result,EF3314 gene was widespread in 24 ...