期刊论文

Immune protective efficacy of recombinant IbeB protein of Pasteurella multocida in mice

中文

中国兽医科学

Chinese Veterinary Science

1673-4696

2017

47

5

630-635

10.16656/j.issn.1673-4696.2017.05.017

湖南省自然科学基金资助项目(14JJ4077)；

本校为第一机构

为了研究猪多杀性巴氏杆菌IbeB重组蛋白(rIbeB)的免疫保护性,根据G enBank中登录的多杀性巴氏杆菌ibeB基因序列设计1对引物扩增ibeB基因,并重组至pET-28a(+)构建重组表达质粒pET- ibeB,转化到大肠杆菌BL21 (D E3),经IPTG诱导获得rIbeB。生物信息学分析表明,猪多杀性巴氏杆菌ibeB基因长1 392 bp,编码蛋白质含463个氨基酸。该蛋白质的理论分子质量为51.98 ku,具有2个外排蛋白结构域,属外膜外排蛋白超家族。蛋白序列比对发现,不同血清型的多杀性巴氏杆菌IbeB的同源性达99%以上。rIbeB诱导小鼠的免疫保护结果表明,第3次免疫后第14天小鼠血清中IgG抗体水平显著升高,明显高于佐剂对照组。攻毒后,对照组...

To explore immune protective efficacy induced by porcine Pasteurella multocida rIbeB, the primers of ibeB gene of P.multocida were designed based on the ibeB gene sequence retrieved from GenBank.The ibeB gene was cloned by PCR and subcloned into pET-28a(+) to construct recombinant plasmid pET-ibeB.The pET-ibeB was then transformed into Escherichia coli BL21(DE3).rIbeB was expressed after being induced by IPTG.The bioinformatics analysis results showed that the ibeB gene consisted of 1 392 bp, encoding 463 amino acids of protein with a theoretical molecular weight of 51.98 ku.The IbeB was an ou...