期刊论文

中文

中国兽医科学

1673-4696

2017

47

2

212-216

10.16656/j.issn.1673-4696.2017.02.013

湖南省战略性新兴产业科技攻关类项目（2016GK4015）；

本校为第一机构

为了解患渗出性皮炎仔猪中猪葡萄球菌内表皮脱落素的流行情况,采集2个猪场（A场和B场）29头患病猪的痂皮、痂皮下渗出液共48份,进行了猪葡萄球菌的分离鉴定及其脱落素基因的检测。结果显示,48份样品中共分离到29株含脱落素基因的葡萄球菌,经生化鉴定和Gap基因测序分析,表明所有菌株均属于猪葡萄球菌。其中,A场和B场分离的猪葡萄球菌脱落素检出率分别为93.3%和64.3%;A场可检出Exh A、Exh C、Exh D和Shet A共4种脱落素基因,B场可检出Exh A、Exh D和Shet A共3种脱落素基因,但Shet A的检出率在2个猪场中均为最高且与其他3种脱落素大量共存,共存率在A场和B场分别为61.5%和70.0%。2个猪场中痂皮源葡萄...

To investigate the prevalence of exfoliative toxins of Staphylococcus hyicus isolated from piglets with exudative epidermitis（EE）.48 samples,crusta and exudate,were collected from 29 piglets with EE on farms A and B.S.hyicus strains were isolated and identified,and the exfoliative toxin genes were detected by PCR.The results showed that 29 Staphylococcus encoding exfoliative toxin genes were isolated,and they were all S.hyicus by biochemical identification and Gap gene sequencing analysis.The positive rate of exfoliative toxin genes in farms ...