期刊论文

Isolation and identification of porcine circovirus type 2 from Hunan Province

中文

中国兽医科学

1673-4696

2019

49

2

213-219

10.16656/j.issn.1673-4696.2019.0025

湖南省教育厅重点项目(15A086) 湖南省自然科学基金面上项目(2018JJ2177) 湖南农业大学校科学基金项目(17QN10).

本校为第一机构

为分离鉴定湖南地区2个猪场的猪圆环病毒2型(PCV2),从临床表现为断奶仔猪多系统衰竭综合征(PMWS)猪体采集组织病料,验证为PCV2感染的病料,用无污染猪肾细胞系(PK15)细胞分离培养。采用间接免疫荧光试验(IFA)、分子克隆及生物信息学等方法对分离病毒进行分析。结果显示,分别获得了2株PCV2:1株为PCV2d(命名为ChenZ-2-1,GenBank号MH718995),另1株为PCV2b(命名为YiY-3-27, GenBank号KU317482),且两分离株的病毒效价TCID_(50)分别为1×10~(5.66)/ mL和1×10~5.5/ mL。本研究有利于提高PCV2的诊断水平,对深入研究PCV2的发病机理及开发PCV2新型疫苗奠定基础。

The purpose of this study was to isolate and identify endemic porcine circovirus type 2 (PCV2) on two farms in Hunan Province.In this study,histopathological materials were carried out from the piglets with post-weaning multi-systemic wasting syndrome(PMWS),and PCV2 in positive samples were propagated and amplified by pollution-free pig kidney cell line(PK15).The isolated virus were further analyzed by different methods,such as indirect immunofluorescence assay(IFA),molecular cloning and bioinformatics analysis.In result,two PCV2 strains were o...