期刊论文

Morphological and molecular markers characteristics ofArgas persicus

中文

中国兽医科学

1673-4696

2019

49

8

990-997

10.16656/j.issn.1673-4696.2019.0115

(SYL201802016):湖南农业大学“双一流”建设项目 (18QN24):湖南农业大学2018年青年科学基金项目

本校为第一机构

为了精准鉴定波斯锐缘蜱,从新疆、甘肃、河北等地采集寄生蜱,借助体视显微镜超景深系统对其形态学特征进行观察;经PCR扩增、测序获得其16S rDNA和cox1基因序列,结合GenBank数据库中参考序列,比对分析同源性并构建系统进化树。结果显示,波斯锐缘蜱幼蜱形态与其他发育阶段差异较大,其体型较小,背、腹表面光滑,无盘窝,生殖孔未形成,足3对。若蜱除生殖孔不显著外,其他形态结构均与成蜱相似。雌、雄成蜱形态学差异主要为:雌蜱生殖孔呈横裂形,雄蜱生殖孔呈半圆形。同时,在16S rDNA和cox1基因序列的邻位相接系统进化树中,波斯锐缘蜱分布于两大进化枝之一的软蜱科,同种聚类良好。结果提示:波斯锐缘蜱具有...

To accurately identify Argas persicus,the morphological characteristics of A.persicus collected from Xinjiang,Gansu and Hebei were observed by the super-depth-of-field system of stereomicroscope. The 16S rDNA and the mitochondrial cytochrome c oxidase subunit 1 gene(cox1) of samples were amplified by PCR and subsequently sequenced.Then the analysis of genetic divergences and Neighbor- Joining(NJ) phylogenetic tree were carried out based on the sequences acquired from A.persicus, combined with the reference sequences from GenBank database.The results showed that the morphology of larva was diff...