期刊论文

Prohibitin 2 is involved in PCV2 infection in PK-15 cells

中文

中国兽医科学

1673-4696

2020

50

4

506-511

10.16656/j.issn.1673-4696.2020.0041

国家自然科学基金项目(31372406) 湖南省教育厅重点项目(15A086) 湖南省自然科学基金面上项目(2018JJ-2177) 湖南省大学生研究性学习和创新性实验计划项目 国家级大学生创新创业训练计划项目(SCX1816) 湖南农业大学“双一流”建设项目(SYL2019048)。

本校为第一机构

为探究Prohibitin2(PHB2)在PCV2感染细胞过程中发挥的作用,首先通过间接免疫荧光试验检测PCV2在PK-15细胞中的增殖变化,同时建立PHB2荧光定量PCR检测体系,然后以PCV2已知的宿主细胞互作蛋白gC1qR为对照,检测PCV2感染PK-15细胞后PHB2 mRNA的动态变化,最后鉴定PHB2 siRNA转染PK-15细胞中PCV2 Cap的表达。结果表明,PHB2在正常及PCV2感染的PK-15细胞中均有效表达。随着PCV2感染PK-15细胞,gC1qR转录水平在病毒感染3 h显著降低,感染6 h时升高,随后(12~ 48 hpi)转录水平下降;而PHB2转录水平在PCV2感染6 h内显著升高,随后(12~48 hpi)mRNA水平有所下降;RNA干扰PHB2对PCV2在PK-15细胞中复制起到显著的抑制...

In order to unveil the role of Prohibitin 2(PHB2) in the process of PCV2 infection,firstly,the PCV2 proliferation in PK-15 cells was detected by indirect immunofluorescence assay.And a real-time quantitative PCR detection method for PHB2 was established,then the gC1qR(known as host cell interaction protein of PCV2) was used as a control to detect the dynamic changes of PHB2 m RNA in PCV2-infected PK-15 cells.Finally,the PCV2 Cap expression in PK-15 cells transfected with PHB2 si RNA was identified by Western-blot.The results showed that PHB2 was efficiently expressed both in normal and PCV2-in...