The pyroxsulam resistance is non‐target site based enhanced herbicide metabolism and/or translocation involving several possible genes (four P450s, one GT, and one ABC transporter) at play in the known glyphosate‐resistant L. rigidum population (NLR70). Abstract BACKGROUND Resistance to multiple herbicides is common in Lolium rigidum. Here, resistance to acetolactate synthase (ALS)‐ and susceptibility to acetyl‐CoA carboxylase (ACCase)‐inhibiting herbicides was confirmed in a glyphosate‐resistant L. rigidum population (NLR70) from Austral...