The toxicity of Cd2+ and its combination with surfactant or simulated acid rain to Vicia faba root tip cell was studied by using micro-nucleus technique. The results showed that the formation of micro-nucleus in Vicia faba root tip cell was strongly induced by Cd2+ in its concentration of 0-10.0 mg.L-1. The micro-nucleus rate was 13.85@1000 at 6.0 mg.L-1Cd2+, 4.53@1000 at 0 mg.L-1Cd2+, and the pollution index (PI) was 3.06. The micro-nucleus ratio and PI decreased when the accompanied surfactant LAS was also presented or the pH values decreased to 4.5 or 3.5. In the meantime, many deformed nuc...