In 2005 and 2007, soil samples were collected from a 27-year experimental field in Wangcheng County of Hunan Province to study the effects of long-term application of chemical fertilizers (CF) and their combinations with pig manure (PM) and rice straw (RS) on the chemical and biochemical properties of reddish paddy soil (Typic Haplic-Stagnic Anthrosis). Under the fertilization, soil pH had somewhat decrease, and soil conductivity changed less, compared with those before the experiment was installed. The contents of soil total organic carbon (TOC), total N, available N and P, and microbial biom...