期刊论文

Genetic Diversity and Relationship of Tea Germplasm Resources Camellia sinensis var. assamica cv. Rucheng Revealed by ISSR Markers

中文

分子植物育种

1672-416X

2017

15

9

3780-3787

10.13271/j.mpb.015.003780

教育部博士点基金项目（20124320110014） 湖南省研究生科研创新项目（CX2016B284）共同资助

本校为第一机构

利用ISSR分子标记技术分析了汝城白毛茶群体的遗传多样性和亲缘关系,结果表明,筛选出的10条重复性好、多态性高的ISSR引物对112个单株进行扩增,共获得190条谱带,其中多态性条带173条,多态性比率达91.05%,显示出汝城白毛茶种群内具有较高的多态性。遗传多样性分析显示,112个株系的有效等位基因数(Ne)介于1.02~2.00之间,平均为1.42;Shannon信息指数(I)介于0.05~0.69之间,平均为0.40; Nei's基因多样性指数(H)介于0.02~0.50之间,平均为0.26;Jaccard相似系数介于0.37~0.83之间,平均为0.60。根据相似系数矩阵按UPGMA法进行聚类,在相似系数为0.42处可将供试的112个汝城白毛茶单株划分为九个类群,其中包...

In this study, the genetic diversity and relationship of tea germplasm resources Camellia sinensis var. assamica cv. Rucheng were studied by using ISSR markers. The results showed that the 10 ISSR primers with high polymorphic, reproducible amplified bands were selected to analyze the genetic diversity and relationship amongst 112 accessions of Camellia sinensis var. assamica cv. Rucheng individuals. A total of 190 bands were obtained, including 173 polymorphic bands and the polymorphic rate had reached to 91.05%, which showed the high polymorphic within Rucheng populations. The analyzing resu...