Sensitive and Direct Detection of Heterodera filipjevi in Soil and Wheat Roots by Species-Specific SCAR-PCR Assays

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成果类型：

期刊论文

作者：

Peng, Huan;Qi, Xiaoli;Peng, Deliang*;Long, Haibo;He, Xufeng;...

通讯作者：

Peng, Deliang

作者机构：

[Qi, Xiaoli; Huang, Wenkun; Peng, Huan; Peng, Deliang; He, Wenting] Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China.

[Long, Haibo] Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Danzhou 571737, Peoples R China.

[He, Xufeng] Hunan Agr Univ, Coll Biosafety Sci & Technol, Changsha 410128, Hunan, Peoples R China.

[He, Xufeng] Hunan Agr Univ, Hunan Prov Key Lab Biol & Control Plant Dis & Ins, Changsha 410128, Hunan, Peoples R China.

通讯机构：

[Peng, Deliang] C

Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China.

语种：

英文

期刊：

PLANT DISEASE

ISSN：

0191-2917

年：

2013

卷：

期：

页码：

1288-1294

DOI：

10.1094/PDIS-02-13-0132-RE

基金类别：

National Key Basic Research Program of China (973 Program)National Basic Research Program of China [2013CB127502]; Special Fund for Agro-scientific Research in the Public Interest [200903040]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31201493]

机构署名：

本校为其他机构

院系归属：

植物保护学院

摘要：

Cereal cyst nematodes are the most important plant-parasitic nematodes on cereal crops in wheat producing areas of the world. Heterodera filipjevi was first reported in China in 2010. In this study, species-specific sequence characterized amplified region polymerase chain reaction (SCAR-PCR) assays for detection and identification of H. filipjevi from infected wheat roots and soil were developed. The species-specific primers were designed according to the randomly amplified polymorphic DNA (RAPD) markers amplified with random primer OPK16. A 646-bp specific fragment of sequence was generated, ...

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Sensitive and Direct Detection of Heterodera filipjevi in Soil and Wheat Roots by Species-Specific SCAR-PCR Assays

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