期刊论文

Liu, Meiling;Liu, Xiaoying

英文

Microchimica Acta

0026-3672

2019

186

2

123

10.1007/s00604-018-3224-5

National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [21645008, 21305042, 21375037]; Scientific Research Fund of Hunan Provincial Education DepartmentHunan Provincial Education Department [14B116]; Science and Technology DepartmentScience and Technology Development Fund (STDF)United States Agency for International Development (USAID)US-Egypt Science and Technology Joint Fund [14JJ4030]; construct program of the key discipline in Hunan province; Aid Program for Science and Technology Innovative Research Team in Higher Educational Institutions of Hunan Province

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Colorimetric methods are described for the determination of ascorbic acid (AA) and alkaline phosphatase (ALP). Both assays are based on the inhibition of the peroxidase (POx)-like activity of Prussian Blue nanocubes (PB NCs) capped with citric acid. They catalyze the oxidation of 3,3,5,5-tetramethylbenzidine (TMB) by H2O2 to produce a blue color with an absorption maximum at 652 nm. On addition of AA, the PB NCs are reduced to Prussian White (PW) which does not act as a POx mimic. This results in a decreased rate of the formation of the blue coloration whose intensity decreases with increasing...