期刊论文

Liu, Meiling;Liu, Xiaoying

英文

Microchimica Acta

0026-3672

2019

186

2

123

10.1007/s00604-018-3224-5

Acknowledgments This work was supported by the National Natural Science Foundation of China (21645008, 21305042, 21375037), Scientific Research Fund of Hunan Provincial Education Department (14B116), Science and Technology Department (14JJ4030), the construct program of the key discipline in Hunan province and the Aid Program for Science and Technology Innovative Research Team in Higher Educational Institutions of Hunan Province. This work was supported by the National Natural Science Foundation of China (21645008, 21305042, 21375037), Scientific Research Fund of Hunan Provincial Education Department (14B116), Science and Technology Department (14JJ4030), the construct program of the key discipline in Hunan province and the Aid Program for Science and Technology Innovative Research Team in Higher Educational Institutions of Hunan Province.

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Colorimetric methods are described for the determination of ascorbic acid (AA) and alkaline phosphatase (ALP). Both assays are based on the inhibition of the peroxidase (POx)-like activity of Prussian Blue nanocubes (PB NCs) capped with citric acid. They catalyze the oxidation of 3,3,5,5-tetramethylbenzidine (TMB) by H2O2 to produce a blue color with an absorption maximum at 652 nm. On addition of AA, the PB NCs are reduced to Prussian White (PW) which does not act as a POx mimic. This results in a decreased rate of the formation of the blue coloration whose intensity decreases with increasing...