期刊论文

Effects of histone methylation and deacetylation on the biosynthesis of huperzine A in the endophytic fungus Colletotrichum gloeosporioides

中文

菌物学报

1672-6472

2019

38

2

242-253

10.13346/j.mycosystema.180242

国家自然科学基金(81773850) 湖南省科技重大专项(2017SK1020).

本校为第一机构

以蛇足石杉Huperzia serrata内生真菌盘长孢状刺盘孢Cg01菌株为研究对象,利用PEG介导的同源重组转化体系,对Cg01组蛋白甲基化酶基因(histone methyltransferases,HMT) CgClr4和组蛋白去乙酰化酶基因(histone deacetylase,HDAC) CgClr3、CgSir2进行基因敲除与回补,并通过实时荧光定量PCR (RT-qPCR)检测了回补株中对应基因表达量以及高效液相色谱HPLC检测突变体菌株中石杉碱甲huperzine A(HupA)产量.结果显示3个基因敲除突变体菌株△CgClr4、△CgClr3、△CgSir2的HupA产量分别为255μg/L、270μg/L、244μg/L,与野生型菌株相比分别下降了21.3％、16.6％、24.7％.在基因回补突变体菌株△CgClr4/CgC...

A histone methyltransferases (HMT) gene CgClr4 and two histone deacetylase (HDAC) gene, CgClr3 and CgSir2, were knocked out and then complemented in the endophytic fungus Colletotrichum gloeosporioides strain Cg01 in Huperzia serrata by the PEG‐mediated genetic transformation system. The expression of the corresponding genes in the complemented strains were detected by RT‐qPCR and huperzine A (HupA) production in the mutant strains were analyzed by high performance liquid chromatography (HPLC). It was found that the HupA production of the 3 g...