期刊论文

Sequencing and phylogenetic analysis of the mitochondrial genome of the “jinhua-jun” isolated from fermented dark tea

中文

菌物学报

1672-6472

2021

40

1

135-151

10.13346/j.mycosystema.200153

Supported by the Hunan Provincial Key Lab of Dark Tea and Jin-hua (2016TP1022), and Undergraduate Innovation and Entrepreneurship Training Program of Hunan City University (S202011527052). Corresponding author. E-mail: liusc@hunau.net ORCID: HU Zhi-Yuan (0000-0003-3774-9448) Received: 2020-05-14, accepted: 2020-07-08

本校为第一机构

本研究对黑茶中优势微生物\"金花菌\"线粒体基因序列进行测定与生物信息分析,并探究其与近缘种微生物的系统发育关系。结果表明,\"金花菌\"线粒体基因组是一条全长77 649bp的环状DNA分子,其ATCG 4种碱基所占比例分别为34.14%、37.64%、15.61%、12.61%,具有显著的A-T偏向性。基因组共编码42个基因(15个蛋白质编码基因,2个rRNA基因和25个tRNA),与近缘种相比缺失了trnT-TGT且多了1个trnM-CAT拷贝,排列顺序上则较为保守。蛋白质编码基因编码频率较高的氨基酸分别为Leu、Ile、Ser、Phe和Val;RSCU频率最高的4个密码子依次是UUA、AUA、UUU和AAU。25个tRNA基因均可形成典型三叶草结构,并存在有35处G-U错...

Sequencing and bioinformatic analysis of the mitochondrial gene sequence of “jinhua-jun (golden flower fungus)”, a dominant microbe in fermented dark tea were conducted. Phylogenetic relationship of “jinhua-jun” with related microbes was analysed. The results showed that the mitochondrial genome of “jinhua-jun” was a circular DNA molecule with total length of 77 649bp, in which A, T, C and G bases were 34.14%, 37.64%, 15.61% and 12.61%, respectively, with significant A-T bias. A total of 42 genes coded 15 PCGs, 2 rRNAs and 25 tRNAs. In co...