期刊论文

中文

菌物学报

1672-6472

2023

42

8

1798-1806

10.13346/j.mycosystema.220490

本校为第一机构

为建立一种快速、简便制备丝状真菌PCR反应模板的方法,提高丝状真菌菌株鉴定与转化子筛选效率,本试验以10种不同种丝状真菌为材料,比较了3种不同试剂微波法制备丝状真菌PCR反应模板的效果,并优化了微波处理的时间。试验结果表...展开更多 为建立一种快速、简便制备丝状真菌PCR反应模板的方法,提高丝状真菌菌株鉴定与转化子筛选效率,本试验以10种不同种丝状真菌为材料,比较了3种不同试剂微波法制备丝状真菌PCR反应模板的效果,并优化了微波处理的时间。试验结果表明,取少量菌丝,加入50 mmol/L NaOH,无菌吸头将菌丝打散,微波炉高火处理30 s,离心取上清,即可获得用作PCR反应的模板。该方法制备的10种...

A rapid and simple method for preparing PCR reaction template of filamentous fungi and improving the efficiency of filamentous fungal strain identification and transformant screening was established. Ten different species of filamentous fungi were used as materials to compare the effects of microwave preparation of filamentous fungi PCR templates using three different reagents, and the duration of microwave treatment were optimized. The templates for PCR could be obtained by adding 50 mmol/L NaOH to a small of mycelium, breaking up the mycelium...