期刊论文

THE PROTECTIVE EFFECTS OF GAMMA-ORYZANOL ON H_2O_2-INDUCED APOPTOSIS IN L02 CELLS

中文

营养学报

0512-7955

2018

40

3

279-284

10.13325/j.cnki.acta.nutr.sin.2018.03.015

湖南省自然科学基金项目（No.2015JJ2077） 湖南省科技计划项目（No.2015NK3008） 湖南省教育厅项目（No.17A098）

本校为第一机构

目的探讨γ-谷维素（gamma-oryzanol,γ-Orz）对过氧化氢（hydrogen peroxide,O2）诱导人肝细胞（L02）凋亡的保护作用。方法 O2建立L02细胞凋亡模型,用不同浓度（0.1,0.2和0.4 mmol/L）γ-Orz对L02细胞进行预处理,检测细胞总抗氧化能力（total antioxidant capacity,T-AOC）与产生羟自由基（OH·）的能力,三磷酸腺苷酶（adenosine triphosphatase,ATPase）活力以及一氧化氮（nitric oxide,NO）水平;Hoechst 33258染色观察细胞形态变化;流式细胞术检测细胞周期变化;琼脂糖凝胶电泳观察细胞凋亡过程中DNA的裂解。结果γ-Orz呈剂量依赖性下调L02细胞产生NO水平,上调OH·的清除能力、ATP酶活力与T-...

Objective To explore the protective effects of gamma-oryzanol(γ-Orz) on H_2O_2-induced apoptosis in L02 cells. Methods The model of apoptosis was established on L02 cells by H_2O_2 treatment. Production of hydroxyl free radicals (OH·), total antioxidant capacity (T-AOC), activity of adenosine triphosphatase (ATPase) and production of nitric oxide (NO) were assayed in L02 cells after pretreatment with γ-Orz at various concentrations (0.1, 0.2 and 0.4mmol/L) in vitro. Meanwhile, the morphological changes of L02 cells were observed by Hoechst 3...