期刊论文

EFFECT OF BETULINIC ACID ON MITOCHONDRIAL APOPTOTIC PATHWAY IN MICE WITH LIVER INJURY INDUCED BY ETHANOL

中文

营养学报

0512-7955

2018

40

6

599-603

10.13325/j.cnki.acta.nutr.sin.2018.06.016

湖南省教育厅项目(No.17A098) 湖南省自然科学基金项目(No.2015JJ2077) 湖南省科技计划项目(No.2015NK3008).

本校为第一机构

目的研究桦木酸(betulinic acid,BA)对酒精性肝损伤小鼠线粒体信号通路的影响。方法56只健康雄性小鼠随机分为8组,即正常对照组(NC),酒精模型组(MC),BA低、中、高剂量组(L-BA,M-BA, H-BA,按0.25、 0.5、1 mg/kg bw给与),BA低、中、高剂量与酒精联合组(L-BAA, M-BAA, H-BAA) 。NC、MC组灌胃1%的可溶性淀粉,其余各组灌胃混悬于可溶性淀粉中不同剂量的BA,1/d,连续14d后,除NC组,L-BA, M-BA,H-BA组灌胃生理盐水外,其余4组均按10 ml/kg灌胃50%酒精诱发酒精性肝损伤模型。检测血清细胞色素P450 2E1(CYP2E1)水平,采用Western blot检测肝脏线粒体信号通路中Bcl-2、Bax、Caspase-9和Caspase-3蛋白表达的变...

Objective To investigate the effect of betulinic acid (BA) on mitochondrial apoptotic pathway in mice with liver injury induced by ethanol. Methods 56 healthy male mice were randomly divided into 8 groups: normal control group (NC), model control group (MC), low-dosage, medium-dosage and high-dosage BA groups (L-BA, M-BA and H-BA, 0.25, 0.5, 1.0 mg/kg bw), low-dosage, medium-dosage and high-dosage BA with alcohol groups (L-BAA, M-BAA and H-BAA). The mice in the NC and MC groups were administered orally with 1% starch and the those in other groups were administered orally with different doses o...