期刊论文

EFFECT OF POLYGONATUM ODORATUM POLYSACCHARIDES ON LIPID METABOLISM IN HIGH-FAT DIET FED MICE

中文

营养学报

0512-7955

2018

40

4

353-359

10.13325/j.cnki.acta.nutr.sin.2018.04.013

湖南省教育厅青年项目（No.168138） 创新平台开放基金项目（No.16K047）

本校为第一机构

目的研究玉竹多糖(POP)对小鼠脂肪代谢关键基因表达的影响,探讨POP干预肥胖形成的机制。方法采用C57BL/6健康清洁级小鼠,每天在喂饲高脂饲料的同时分别经口灌胃给予奥利司他(30 mg/kg·d)和玉竹多糖(400 mg/kg·d),期间跟踪小鼠能量摄入量和体质量变化,8 w实验结束后检测小鼠脂肪和肝脏湿重,测定血清血脂含量,采用qPCR技术检测小鼠肝脏脂代谢关键基因表达水平。结果与高脂模型组相比,玉竹多糖能明显抑制小鼠体质量增加,显著降低Lee's指数、身体质量指数、脂肪和肝脏系数、血清甘油三酯和低密度脂蛋白浓度(P

Objective To study the influence of polygonatum odoratum polysaccharides （POP） on the expression of key genes in lipid metabolism and explore the mechanism in obese. Methods C57BL/6 mice were fed a high fat diet and given orlistat（30 mg/kg·d） or POP（400 mg/kg·d） by gavage every day at the same time. After 8 weeks, body weight, energy intake, wet weights of liver and fat tissues, and levels of serum lipid profiles were detected. The mRNA expression of key genes in lipid metabolism were also analyzed using quantitative real-time polymeras...