期刊论文

中文

中国烟草科学

1007-5119

2008

29

6

23-26

10.3969/j.issn.1007-5119.2008.06.006

国家烟草专卖草局项目“高香气优质烟叶开发“（2001B10）；

本校为第一机构

为探索外加酶制剂施于调制后原烟发酵过程的效果，设计了不同加酶量、作用时间、相对湿度和温度处理的方法来降解烤烟烟叶中的淀粉和蛋白质的试验。结果表明，单一施用中性蛋白酶对烤烟蛋白质的降解率不理想，综合使用α-淀粉酶、葡萄糖淀粉酶针对不同的部位均有不同的相对最佳处理组合。在各等级相对最佳条件下施加α-淀粉酶、葡萄糖淀粉酶后再喷施中性蛋白酶发酵烟叶后针对3个等级烟叶的淀粉和蛋白质有所降解，氨基酸含量均有显著提高，但还原糖含量、总氮和烟碱含量变化不明显。

In order to explore the effect of added enzyme preparation on fermentation of flue-cured tobacco, an experiment on degradation of starch and protein in tobacco leaves was conducted by using various treatments of added enzyme level, time, RH and temperature. The results showed that single application of neutral protease could not achieve favorable degradation rate of protein in flue-cured tobacco, while the best combinations of a-amylase and glucoamylase were found for different stalk position leaves. Under the relatively optimum conditions, three grades tobacco leaves treated by a-amylase, glu...