期刊论文

Isolation and Identification of a Benzoic Acid-degrading Bacterium and Its Effect on Growth of Flue-cured Tobacco

中文

中国烟草科学

1007-5119

2016

37

1

72-77

10.13496/j.issn.1007-5119.2016.01.013

中国烟草总公司项目“土壤微生物生态修复关键技术研究与应用”（11020100219） 湖南省研究生科研创新项目“基于烤烟土壤-根系互作机制的烟叶钾素调控技术研究”（CX2015B238）

本校为第一机构

为降低烟草连作对烟叶产量和品质以及烟草生长发育带来的不良影响,通过平板筛选和摇床复筛,从烤烟根系分泌物中筛选出一株能高效降解苯甲酸的菌株。结果表明,根据rDNA-ITS的扩增及序列分析、培养特征及形态特征观察初步鉴定该菌株为黄曲霉(Aspergillus flavus);该菌株培养15 d内,在0~800 μmol/L苯甲酸浓度范围内能够正常生长,800~1600 μmol/L苯甲酸浓度范围内生长受到一定程度抑制;该降解菌在以苯甲酸为唯一碳源的800 μmol/L浓度LBA培养基中菌株干重、降解率均达到最大,菌株干重为0.765 g,降解率为19.98%;菌株在培养至第9天时降解速率达到最快,为8.5 mg/d;烤烟幼苗生长过程中施加降解菌液能在...

In order to reduce adverse effects of continuous cropping on yield and quality to some extent, as well as growth and development of flue-cured tobacco, the authors isolated a benzoic acid decompositon strain from the root exudate of tobacco by plate screening and shaker rescreening. The strain was identified asAspergillus according to rDNA-ITS and sequence analysis, culture characteristics and morphological characteristics. The results showed that the strain could grow normally in the 0-800μmol/L benzoic acid concentration range in 15 days. To...