期刊论文

Identification and Fermentation Optimization of Antagonistic Bacillus amyloliquefaciens Xe01

中文

中国烟草科学

1007-5119

2020

41

6

58-67

10.13496/j.issn.1007-5119.2020.00.153

中国烟草总公司湖南省公司科技项目“防治烟草主要根茎病害的生物型菌肥研制与应用”(17-20Aa01) 湖南省自然科学基金项目“短短芽孢杆菌B011抗真菌天然产物的纯化鉴定及其编码基因/基因簇的克隆”(2019JJ40119) 湖南省重点研发计划项目“湖南优势种植业功能微生物种质资源挖掘、选育与应用”(2019NK2192)。

本校为第一机构

为获得对烟草青枯病菌具有拮抗作用的生防菌,从湖南永州青枯病发病较重烟田的健康烟株根系中分离获得1株对烟草青枯病菌具有较强抑菌活性的菌株,对峙培养其抑菌圈直径达到30.5 mm,将其命名为Xe01。利用形态观察、生理生化特征及16S rRNA基因序列分析鉴定其为解淀粉芽孢杆菌(Bacillus amyloliquefaciens),温室盆栽和小区试验中Xe01对烟草青枯病的防治效果分别达到56.32%和61.46%。对其发酵培养基及发酵条件进行单因素试验及正交设计优化,最适培养基碳源、氮源和无机盐种类及浓度为葡萄糖9.0 g/L、牛肉膏3.0 g/L、氯化钙4.0 g/L;最佳发酵条件为pH 7.0,30℃,180 r/min,培养52 h,使用优化后的发酵条...

To obtain antagonistic biocontrol bacteria for tobacco bacterial wilt control,a strain(Xe01)with strong antibacterial activity against Ralstonia solanacearum was isolated from the roots of healthy tobacco plants in Yongzhou,Hunan Province.It was found that the diameter of inhibition zone was 30.5 mm by confrontation culture method,and the strain was identified as Bacillus amyloliquefaciens by morphological observation,physiological and biochemical characteristics and 16S rRNA gene sequence analysis.The control effect of antagonistic bacterium X...