期刊论文

中文

中国烟草科学

1007-5119

2023

44

03

10-15

10.13496/j.issn.1007-5119.2023.03.002

HN2021KJ02:中国烟草总公司湖南省公司科技项目 HN2021KJ04:中国烟草总公司湖南省公司科技项目

本校为第一机构

为了探究低温胁迫诱导烟草早花的分子机制，以低温敏感品种云烟87为试验材料，在12℃低温条件下处理烟苗14 d，取茎顶端组织进行烟草开花调控基因分析，并调查统计了烟株中心花开放时间和叶数。结果显示，与对照相比，低温胁迫导致烟株中心花开放时间平均提前7 d，叶数平均减少5片。基因表达分析结果表明，低温胁迫下开花调控基因NtMADS4,NtMADS11,NtAP1,NtSOC1,NtFUL,NtNFL1,NtFT1,NtFT3,NtFT4,NtFT5表达水平明显高于对照，在正常环境中恢复生长7～14 d，表达量下降至与对照相近的水平。以上结果表明，低温诱发烟草早花可能与这些基因上调表达有关。

To investigate the molecular regulatory mechanism of low temperature induced early flowering,a cold-sensitive tobacco cultivar Yunyan 87 was treated with cold stress of 12℃for 14 days.The shoot apical tissues of plants were collected for flowering-regulatory gene expres...MORE To investigate the molecular regulatory mechanism of low temperature induced early flowering,a cold-sensitive tobacco cultivar Yunyan 87 was treated with cold stress of 12℃for 14 days.The shoot apical tissues of plants were collected for flowering-regulatory gene expres...