In order to enhance cellulase-producing capacity of Bacillus 25-2, response surface method was applied to optimize its fermentation conditions. Firstly, two level factorial design of Plackett-Burman was used to evaluate the effects of six related factors on cellulase activity. Three factors including fermenting time（X1）, fermentation temperature（X2） and initial pH value（X3）, as the significant factors influencing cellulase activi- ty, were screened out, Then the path of steepest ascent experiments were adopted to approach the optimal regi...
