Previous single factor test results indicated that temperature, pH value and inoculating quantity were the key factors influencing cellu- lase yield of Photon putaminura LYYZ90-19. Based on previous study, response surface method was applied to construct two degree polynomial regression models of the three key factors with cellulase activity as response values, and Design Expert software was used to optimize these mod- els. The results showed that the optimal conditions were as follows: temperature, pH and inoculating quantity were 27.4 ℃, 6....