期刊论文

中文

中国植保导刊

1672-6820

2018

38

7

5-12

10.3969/j.issn.1672-6820.2018.07.001

国家重点研发计划项目（2016YFD0300700） 湖南省“十二五”重点学科项目（0904） 湖南省研究生科研创新项目（CX2016B303）

本校为第一机构

从湖南桃江石牛镇贺家塘村稻田感稻瘟病品种T优535的病稻丛中,选取健康的稻株和根际土样,用稀释分离法分离得到菌株464株。采用平板对峙法,筛出1株来自根际土壤的RS39菌株,该菌株能够高效地抑制稻瘟病菌生长,其菌丝生长抑制率达89.62%。经形态观察、生理生化及16S r DNA序列分析,表明RS39菌株为枯草芽孢杆菌（Bacillus subtilis）。根据菌株RS39对10种植物病原菌的抑菌谱测定数据显示,该菌株对8种真菌病原物有抑菌作用（42.42%-75.26%）,对2种细菌病原物无抑菌效果。

To explore and utilize bio-control resources for controlling rice blast, 464 strains were isolated from healthy rice plants and rhizosphere soil located in paddy field of susceptible cultivar T You 535 at Hejiatang Village, Shiniu Town,Taojiang Country in Hunan by method of plate dilution. Strain RS39 with the strongest antagonistic activity against the blast fungus among above-mentioned 464 strains was obtained, which showed inhibition activity on mycelium growth of Magnaporthe oryzae（89.62%）. On basis of morphological analysis, physiologica...