A bacterial strain B30 of degrading 12 alkyl-polyoxyethylene (Brij-30) was isolated, purified and sorted from the wastewater in pollution-outlets of a factory, and identified as Pandoraea sp.. At temperature of 30°C, pH of 5~8, Brij-30 concentration lowered 0.20g/L, protein peptone as the nitrogen source, bacterial strain B30 degradation effect was the best. ZnO2+, Ca2+, Al3+, Fe3+ had greater promoting effect on the growth of bacterial strain B30 and its degradation property. At low concentration area (0.05~0.20 g/L), bacterial strain B30 gre...