期刊论文

Hu, H.

中文

中国环境科学

1000-6923

2008

28

1

43-48

10.3321/j.issn:1000-6923.2008.01.010

本校为第一且通讯机构

从洗涤剂厂排污口附近的河水中，分离纯化到2株能分别以壬基苯基聚氧乙烯醚（Triton X-100）和十二烷基聚氧乙烯（23）醚（Brij-35）为唯一碳源和能源生长的降解菌株，分别命名为菌株T-1和B-2．根据菌落特征、菌体形态、生理生化反应分析以及16S rDNA序列分析，确认菌株T-1为苍白杆菌属（Ochrobactrum sp．），菌株B-2为伯克霍尔德菌属（Burkholderia）．在底物浓度较低（50～100mg／L）时，Triton X-100降解速度〈Brij-35；在底物浓度较高（200～1500mg／L）时,Triton X-100的降解的速度〉Brij-35．T-1在pH6-8，B-2在pH7时生长最好；最适生长温度分别为25，30℃．2株菌对氮源利用广泛．Zn~(2＋...

Two nonionic surfactant-degrading bacteria designated as T-1 and B-2 were isolated from the surfactant-polluted water by repeated subculture in liquid medium with Triton X-100 and Brij-35 as the sole carbon and energy source respectively. Strain T-1 and B-2 were identified as Ochrobactrum sp. and Burkholderia, according to their characteristics, shapes, physiochemical and the analyse of the phylogenetical analyses of 16S rDNA sequence. A significant positive relationship was found between bacterial growth and concentration of nonionic surfactant degradation. In a mineral salts medium with 50-1...