期刊论文

Yang, Hai-Jun(1227677453@qq.com)

中文

中国环境科学

1000-6923

2016

36

8

2444-2452

10.3969/j.issn.1000-6923.2016.08.026

2015JJ2079:湖南省自然科学基金 K1403022-31:长沙市科技计划

本校为第一且通讯机构

为提高辛基酚聚氧乙烯醚（OPn EO）的生物降解效果,在本实验室已筛选出的H1、TXBc10、OPQb11、TXBa23四株OPn EO高效降解菌的基础上,首次从构建OPn EO混合菌的角度,着重探究了四菌株等比例不同组合降解OPn EO的效果.结果表明,混合菌L9（H1∶TXBc10∶TXBa23为1∶1∶1）培养7d后对初始浓度500mg/LOPn EO的降解率最高,达到56.44%,比各单一菌株降解效果有较明显提高.运用单因素试验考察了影响L9的相关因素,初步确定L9降解OPn EO的最适外加碳源和氮源分别为葡萄糖和胰蛋白胨,最适初始p H值为7.0,最适温度为28℃,最适接种量为4%.Plackett-Burman试验筛选获得影响OPn EO降解率的3个显著因子为L9接种量、...

In order to heighten the biodegradation effect of the non-ionic surfactant octyl phenol ethoxylates (Octylphenyl polyethoxylates, OPnEO) in the laboratory, four mixed degradation bacteria (H1, TXBc10, OPQb11, TXBa23) were screened to degrade OPnEO. The construction of OPnEO mixed flora centers on the degradation effects of four bacterial strain of different ratios on OPnEO. The degradation rate of hybrid strain L9 (H1:TXBc10:TXBa23 1:1:1) to OPnEO reached 56.44% (highest), slightly higher than the degradation effect of each single strain. The factors related to degradation efficiency of L9 wer...