期刊论文

Effects of long-term fertilization on the abundance of the key denitrifiers in profile of paddy soil profiles

Xu, H.-Q.

中文

中国环境科学

1000-6923

2019

39

5

2154-2160

10.19674/j.cnki.issn1000-6923.2019.0257

41771300:国家自然科学基金 41301274:国家自然科学基金

本校为第一且通讯机构

为了探讨长期施肥对稻田不同土层关键反硝化功能种群丰度的影响及核心驱动因子,以湖南宁乡长期施肥定位试验田为平台,选取不施肥(CK)、全量化肥(NPK)和秸秆还田(ST)3个处理,结合实时荧光定量PCR(qPCR)技术,系统分析了稻田不同土层(0~10,10~20,20~30,30~40cm)关键反硝化功能基因(narG、nirK和nirS)的丰度及其与土壤理化性质的内在联系.结果表明,相比于不施肥处理(CK),施肥处理(NPK和ST)在0~40cm土层土壤SOC、TN、 NO_3~- -N、NH_4~+-N和Olsen-P 分别显著增加了2.2%~83.6%,3.5%~58.3%,70.8%~222.1%,0.9%~83.7%和16.5%~94.5%,pH 值下降了0.31~0.67个单位;长期施用化肥和秸秆使narG、nirK和nirS基因...

The aims of this study were to explore the effect of long-term fertilization on the abundance of the key denitrifiers in paddy soil profiles (0~40cm), and the core factors driving denitrifiers. Soils with non-fertilization (CK), inorganic fertilizer (NPK) and organic fertilizer (ST) were collected in Ning xiang County, Hunan Province, and real-time fluorescent quantitative PCR technology was used to analyze the abundance of narG-, nirK- and nirS-containing communities in paddy soil profile (0~10cm, 10~20cm, 20~30cm, 30~40cm) and their relations...