Preparative isolation and purification of theaflavins and catechins by high-speed countercurrent chromatography

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期刊论文

作者：

Wang, Kunbo;Liu, Zhonghua*;Huang, Jian-an;Dong, Xinrong;Song, Lubing;...

通讯作者：

Liu, Zhonghua

作者机构：

[Song, Lubing; Huang, Jian-an; Liu, Zhonghua; Pan, Yu; Wang, Kunbo; Dong, Xinrong; liu, Fang] Hunan Agr Univ, Minist Educ, Key Lab Tea Sci, Changsha 410128, Hunan, Peoples R China.

[Song, Lubing; Huang, Jian-an; Liu, Zhonghua; Pan, Yu; Wang, Kunbo; liu, Fang] Hunan Agr Univ, Dept Tea Sci Hort, Changsha 410128, Hunan, Peoples R China.

[Song, Lubing; Huang, Jian-an; Liu, Zhonghua; Pan, Yu; Wang, Kunbo; liu, Fang] Hunan Agr Univ, Dept Tea Sci Hort, Landscape Coll, Changsha 410128, Hunan, Peoples R China.

[Liu, Zhonghua] Hunan Agr Univ, Minist Educ, Key Lab Tea Sci, Furong Dist, Changsha 410128, Hunan, Peoples R China.

通讯机构：

[Liu, Zhonghua] H

Hunan Agr Univ, Minist Educ, Key Lab Tea Sci, Furong Dist, Changsha 410128, Hunan, Peoples R China.

语种：

英文

关键词：

High-speed countercurrent chromatography;Theaflavins;Black tea;Catechins;Green tea

期刊：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

ISSN：

1570-0232

年：

2008

卷：

867

期：

页码：

282-286

DOI：

10.1016/j.jchromb.2008.04.005

机构署名：

本校为第一且通讯机构

院系归属：

园艺园林学院

摘要：

High-speed countercurrent chromatography (HSCCC) has been applied for the separation of theaflavins and catechins. The HSCCC run was carried out with a two-phase solvent system composed of hexane-ethyl acetate-methanol-water-acetic acid (1:5:1:5:0.25, v/v) by eluting the lower aqueous phase at 2 ml/min at 700 rpm. The results indicated that pure theaflavin, theaflavins-3-gallate, theaflavins-3′-gallate and theaflavin-3,3′-digallate could be obtained from crude theaflavins sample and black tea. The structures of the isolated compounds were positively confirmed by 1H NMR and 13C NMR,...

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Preparative isolation and purification of theaflavins and catechins by high-speed countercurrent chromatography

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