期刊论文

中文

中国预防兽医学报

1008-0589

2010

32

3

205-209

(06CA06):湖南省教育厅科技科研项目 (06FJ4101):湖南省科技厅科技科研项目

本校为第一机构

为了给表达高活性的抗菌肽Hadrurin提供开发应用的依据,进一步为用基因工程方法生产具有多种生物活性的抗菌肽Hadrurin蛋白提供研究基础,本研究表达获得重组抗菌肽Hadrurin（rHadrurin）蛋白的基础上,将纯化的rHadrurin蛋白进行肠激酶酶切,恢复其天然活性结构。用最小抑菌浓度（MIC）和最小杀菌浓度（MBC）方法检测抗菌肽Hadrurin在不同剂量、不同pH值、不同保存温度下对鸡致病性大肠杆菌和金黄色葡萄球菌的抑菌活性及杀菌活性。并以小鼠为动物模型,进行抗菌肽rHadrurind对小鼠的体内保护实验。结果表明抗菌肽rHadrurin对上述细菌的最小抑菌范围为1.32μg/mL～4.32μg/mL,最小杀菌范围为1.77μg...

Hadrurin was originally found in the venom of the Mexican scorpion Hadrurus aztecus and has demonstraed antimicrobial activity against a wide range of bacteria. In this study,Hadrurin was expressed in E.coli BL21 from the recombinant expression plasmid pET-H. The protein was purified by affinity chromatography,and digested with enterokinase to restore its active natural structure. Its bactericidal and bacteriostatic activity against avian pahtogenic E.coli and Staphylococcus aureus under various pH values and storage temperatures were tested by...