期刊论文

中文

中国预防兽医学报

1008-0589

2010

32

10

815-817

10.3969/j.issn.1008-0589.2010.10.17

湖南省教育厅科技项目（09C1209） 湖南省教育厅重点项目（06A028） 湖南省养殖项目（200813）

本校为第一机构

本研究旨在阐明边缘无浆体（A.marginale）MSP4基因的遗传变异情况。应用PCR扩增A.marginale虫株的MSP4基因的部分序列（pMSP4）,将其克隆至pGEM-T载体,重组质粒经菌落PCR鉴定及序列分析,结果表明来自湖南省的6株边缘pMSP4的序列长度均为530bp,与GenBank中登录的A.marginale相应序列相似性在98.3%以上,与其它无浆体的差异大于9.7%。由于A.marginale pMSP4序列种内相对保守,种间差异较大,因此,可作为种间遗传变异研究的标记。本研究为A.marginale的分子流行病学调查等提供了实验依据。

The objectives of the present study were to examine sequence variation in the major surface protein 4（MSP4） gene of Anaplasma marginale isolates from Hunan province.The partial MSP4（pMSP4） sequences of 6 A.marginale isolates were amplified and amplicons were cloned and sequenced.The pMSP4 gene was 530 bp in length and shared 98.3% sequence identity with that of the A.marginale in GenBank.There was no significant variation in pMSP4 sequences within A.marginale,however the inter-species variation was obvious.The results indicated that the pMS...