期刊论文

Identification, virulent factors detection and pathogenesis investigation of capsule serotype F Pasteurella multocida isolated from pigs

中文

中国预防兽医学报

Chinese Journal of Preventive Veterinary Medicine

1008-0589

2017

39

3

210-214

10.3969/j.issn.1008-0425.2017.03.10

家畜疫病病原生物学国家重点实验室开放基金课题（SKLVEB2013KFKT002）； 湖南省战略性新兴产业科技攻关项目（2016GK4015）；

本校为第一机构

为调查多杀性巴氏杆菌(P.multocida)在湖南猪群中的流行情况,本实验室于2015年6月~2016年3月在湖南大型屠宰场采集猪肺脏222份和猪扁桃体180份临床样品,采用血琼脂培养和PCR方法进行P.multocida的分离鉴定,得到4株F型P.multocida。对分离的4株F型P.multocida进行毒力基因检测以及小鼠的致病性研究,结果显示4株菌均含有 ptfA、pthA、fimA、 sodA、 sodC、ompA、 ompH、oma87、plpB、 exbB、exbD、tonB、hgbB、Fur、 nanH、 hsf-1和 hsf-2毒力基因,但 tadD、toxA、 hgbA、 nanB. pmHAS、 tbpA毒力基因均为阴性;4株菌致病性试验结果显示,感染小鼠的剂量分别为4 cfu/只、5 cfu/只、11 cfu/只和17 cf...

Four Pasteurella multocida capsule serotype F strains were isolated in this lab by using blood agar and PCR method from 222 lungs and 180 tonsils of slaughtered pigs in Hunan province during June 2015 to March 2016. 23 virulence-associated genes harboured in those 4 isolates were positive of virulence-associated genes of ptfA, pfhA, fimA, sodA, sodC, ompA, ompH, oma87, plpB, exbB, exbD, tonB, hgbA, Fur, nanH, hsf-1 and hsf-2 detected by PCR. Pathogenic test showed that all of the mice died after 60 hrs when inoculated intraperitoneally with the isolates at dose of 4 cfu, 5 cfu, 11 cfu and 17 c...