期刊论文

Detection and analysis of gelatin liquefaction phenotypes and five virulence genes of Enterococcus isolated from swine

中文

中国预防兽医学报

1008-0589

2019

41

4

361-365

10.3969/j.issn.1008-0589.201808034

湖南省科技计划项目(2015NK3017).

本校为第一机构

为探究明胶液化的表型与其5种毒力基因在不同来源和不同种属猪肠球菌中的分布差异,本研究采用PCR方法以及明胶液化试验对湖南分离的375株肠球菌携带的明胶酶基因(gelE)、调控gelE表达的毒力基因反应调节子基因(fsrA)、前肽加工蛋白基因(fsrB)、组氨酸激酶基因(fsrC)、丝氨酸蛋白酶基因(sprE)共5种毒力基因的分布情况以及液化明胶现象进行检测。结果显示,共263株肠球菌检测到了毒力基因,检出率分别为41.3 %(gelE)、45.9 %(fsrA)、50.9 %(fsrB)、48.8 %(fsrC)、48.8 %(sprE)。能够同时检测到5种毒力基因的菌株共有110株,其中粪肠球菌106株,且该5种毒力基因在106株粪肠球菌中的检出率均为最高,另外...

In order to explore the distribution of gelatin liquefaction phenotypes and five virulence genes in the Enterococcus isolated from different sources and different breeds of swine, five virulence genes and gelatin liquefaction reaction were detected and examined among 375 Enterococcus isolated from swine in Hunan province by PCR and gelatin liquefaction tests. The results showed that virulence genes were detected in 263 Enterococcus isolates and the detection rates were 41.3% for gelE (gelatinase gene), 45.9% for fsrA (esponse regulator gene), 50.9% for fsrB (propeptide processing protein gene)...