期刊论文

Study of the immunoprotective efficacy of recombinant outer membrane carry protein LOLA of Actinobacillus pleuropneumonia in mice

中文

中国预防兽医学报

1008-0589

2020

42

6

607-611

10.3969/j.issn.1008-0589.201909014

湖南省教育厅重点项目(16A101) 湖南省自然基金(2017JJ2116)。

本校为第一机构

为筛选出具有广谱抗猪传染性胸膜肺炎作用的疫苗候选分子,本研究利用PCR技术从血清1型猪传染性胸膜肺炎放线杆菌(App)基因组克隆外膜蛋白(Omp)lola基因。经生物信息学分析显示,App lola基因由633 bp组成,编码210个氨基酸,不同的血清型之间具有很高的同源性。将App lola克隆至pET-32a(+),转化大肠杆菌,表达重组蛋白rLOLA。取4周龄小鼠60只,随机分为6组,分别于0、2、4周经皮下免疫:弗氏不完全佐剂(IFA)+10 μg rLOLA(低剂量组)、IFA+30 μg rLOLA(中剂量组)、IFA+50 μg rLOLA(高剂量组)、30 μg rLOLA(rLOLA组)、IFA(对照组)和生理盐水(对照组)。ELISA检测结果显示,第3次免疫接种后相较于对照组(...

In order to screen candidate vaccines with broad-spectrum and disease-resistant effects, the outer membrane protein(Omp)lolagene was amplified by PCR from genome of Actinobacillus pleuropneumoniae(App)sera type I.Bioinformatics analysis revealed that the OmpLolA consists of 633 bp, encoding 210 amino acids.Homology analysis showed that the sequence shared high similarity between different serotypes of App.In this study, the App lola was inserted into pET-32a(+)vector and then transformed into Escherichia coli BL21 to express the recombinant pro...