期刊论文

中文

中国预防兽医学报

1008-0589

2023

45

11

1165-1171

10.3969/j.issn.1008-0589.202304011

2017JJ2116:湖南省自然科学基金项目

本校为第一机构

为评估猪多杀性巴氏杆菌（Pm）毒力蛋白Dot诱导小鼠产生的免疫保护效果，本研究根据GenBank登录的A型Pm CS株dot基因序列设计特异性引物，以CS株基因组DNA为模板经PCR扩增dot基因片段，PCR产物测序后利用生物信息学软件进行序列分析。结果显示，dot基因长729 bp，编码含242 aa的膜蛋白；该蛋白的N端有一个24 aa组成的信号肽，具有重复Sel 1结构功能域，属于四肽重复超家族；抗原表位分析显示，Pm Dot蛋白含有6个抗原表位；序列同源性分析显示，不同血清型Pm Dot蛋白氨基酸序列的同源性达99%以上。将dot基因克隆至原核表达载体p ET-28a（+）中构建重组表达质粒p ET-dot，转化大肠杆菌BL21 （DE3）...

To evaluate its immune protective efficacy of the virulent Dot protein of Pasteurella multocida(Pm)in mice,a pair of specific primers were designed based on the dot gene sequence of Pm CS strain in GenBank,the dot gene fragment was amplified by PCR from genomic DNA of Pm serotype A CS strain.Sequencing and bioinformatics analysis of the products showed that the dot gene consisted of 729bp and encoded a membrane protein containing 242 amino acids.The N-terminus of this protein had a signal peptide composed of 24 amino acids,with a repeat sel 1 d...