期刊论文

中文

湖北农学院学报

1004-3888

2001

21

02

164-166

湖南省科技厅资助项目! （98- 2 5 - 3）；

本校为第一机构

采用聚阳离子复合物 ,用于过氧化氢酶与酵母细胞的共固定化 ,可防止使用过程中酶的渗漏 ,改善了供氧效果和酵母的增殖。固定化酶的最适反应 pH值范围为 68。当聚阳离子浓度达 1 50mmol/L时 ,固定化酶活力回收可达 68.6% ,在室温条件下 ,5d时酶活力下降58%。这种酶和酵母细胞的共固定颗粒 ,在 1d时间内使颗粒内酵母细胞增殖至 50亿 /g。

Catalase and yeast were immobilized together by polycation complex.Catalase leakage was overcome in the doing so. Oxygen supply and yeast multiplication were improved. The optimum pH of the immobilizing was 6～8.The activity recovery of the immobilized enzyme reached 68% as polycation concentration was kept at 150 mmol/L.At room temperature,the activity of enzyme decreased about 58% in the 5th day.In the co-immobilizing particle...