The aim of the present study was to establish a fourplex RT-PCR method for examining the infecting peppers to improve the efficiency of pepper virus detection, which includes Tobacco mosaic virus (TMV), Cucumber mosaic virus (CMV), Broad bean wilt virus- 2 (BBWV-2) and Potato virus Y (PVY). According to the genome sequences of TMV, CMV, BBWV-2 and PVY published by GenBank, four specific pairs of primers were designed to verify the specificity and sensitivity of detection system by simplex and multiplex RT-PCR method. In addition, using set multiplex RT-PCR method pepper plant samples of two ge...
